Table 5.

Optimization of DNase I enzyme concentration withAvaI restriction enzyme

ControlCT value (ΔRn value) with the following DNase I concn (U/liter):
30252016.6106.6No enzyme
Positive, E. coli 24.90 (0.4)23.20 (0.8)21.60 (1.4)16.44 (2.0)16.4417.416.35 (3.0)
Negative>45.0a >45.0>45.0>45.030.129.328.4
  • a A CT value of >45.0 is deemed to be negative.