Table 4.

Similarity matrix of seven Malassezia species on the basis of PCR-REA types of the LSU and ITS regions

MalasseziaspeciesMultilocus genotypeaSimilarity Indexb with:
M. pachydermatisM. sympodialisM. slooffiaeM. obtusaM. globosaM. restricta
M. furfur 1010001000.770.550.550.550.330.33
M. pachydermatis 0110001000.330.330.770.550.55
M. sympodialis 1000010010.550.330.330.33
M. slooffiae 1001000100.550.550.55
M. obtusa 0110000100.770.77
M. globosa 0100100101.00c
M. restricta 010010010
  • a PCR-REA types that resulted from the analysis of the LSU of the rRNA gene and the ITS have been used to construct these multilocus genotypes. Only the common representative PCR-REA types for each Malassezia species are included. The sequence of PCR-REA types is ABCC′DEFGH; 1 represents the presence and 0 represents the absence of a particular PCR-REA type.

  • b Similarity indices are calculated on the basis of the number of matches between all pairs ofMalassezia species. Either the presence or absence of a particular PCR-REA type is considered a match.

  • c M. globosa and M. restricta could be distinguished only by TD-PCR, using primers for the β-tubulin gene.