Table 1.

Characteristics of virus strains used in this study

StrainIC50 (μg/ml) for:TKIsolationReference(s)
ACVaPAAaActivitybMutationcSizedPhenotype
HSV-1
 VR-30.08819.511.8376WT36, 37
 VRTK >10052.11.6R281Stop280TK In vitro from the VR-336, 37
 KOS0.01213.712.3376WT
 KOSdlsactk>10023.31.34-bp del. at codon 149 and 150180TK In vitro from the KOS3
 SC160.107.713.0376WT
 SC16 S146.119.83.1C336Y376TKA In vitro from the SC166
 DM21>10035.71.2816-bp del.TK In vitro from the SC167
 TAS0.08910.512.7376WT2, 31
 TAR>10011.41.3One-nucleotide del. at codon 355407TK In an immunodeficient child from the TAS31
 BW-S0.1412.913.0376WT34
 BW-R35.313.41.8One-nucleotide ins. at codon 185227TK In an immunodeficient child from the BW-S34
 KH520.05611.112.0376WT17
 KH540.0429.013.4376WT17
 WT510.05228.411.9376WT17
 11334>10015.30.9One-nucleotide del. at codon 146181TK From an AIDS patient12
HSV-2
 UW2680.675.34.5375WT36, 37
 UWTK 56.214.21.1One-nucleotide ins. at codon 186228TK In vitro from the 28636, 37
 1860.4323.55.8376WT
 87020.6719.77.7376WTFrom an AIDS patient8
 870855.024.1NTR223H376TKA From an AIDS patient8
 8713>10013.51.3One-nucleotide del. at codon 7385TK From an AIDS patient8
 11571>10021.71.2One-nucleotide ins. at codon 266390TK From an AIDS patient12
 11572>10027.71.0D274G376TK From an AIDS patient12
 11575>10027.4NTOne-nucleotide ins. at codon 186228TK From an AIDS patient12
 1178532.825.41.4N78D, R223H376TK From an AIDS patient12
 4365-966.427.66.8N78D, L140F, R177W, R220K375TKA From a healthy adult14
VZV
 YS0.657.064.6341WT15, 33
 YSR13.54.71.2One-nucleotide del. at codon 164170TK In vitro from the YS15, 33
Cell (HEL)74.148.21.0
  • a The 50% inhibitory concentration (IC50) of ACV and phosphonoacetic acid (PAA) was determined by a plaque reduction assay on HEL fibroblasts.

  • b Thymidine kinase activity in mock- or virus-infected HEL fibroblasts at 8 h postinfection was measured and expressed as fmol/cell/min.

  • c The mutation sites of the BW-R, 11334, 11571, 11572, 11575, and 11785 were determined in this study, and other data are cited from previous reports which listed in the “Reference(s)” column.

  • d Predicted size of the TK polypeptides from the nucleotide sequence of the TK gene were expressed as the number of amino acids.