Table 3.

DNase I concentration optimization

DNase I source and controlCT value (ΔRn value) with DNase I at the following concn (U/liter):
100302520105No enzyme
Promega
 Positive>45.0>45.023.6524.0427.7126.88 (0.35)23.5 (1.4)
 Negative>45.0a >45.031.8028.8131.79>45.028.93
Life Technologies
 Positive29.4025.2724.4924.42 (1.4)23.31 (3.0)
 Negative>45.031.6729.7431.1930.07
  • a A CT value of >45.0 is deemed to be negative.