Table 3.

Molecular differentiation of Malasseziaspecies by PCR-REA

Genomic regionRestriction endonucleasePCR-REA types (Malassezia speciesa)
26S rRNA gene (LSU) AvaIA (Mf, Msy, Msl); A′ (Mf,Msl); B (Mg, Mr, Mo,Mp)
TS (ITS 1-ITS 4) EcoRIC (Mf, Mp, Mo); C′ (Msl); D (Mg, Mr); D′b, E (Msy)
NcoIF (Mf,Mp); G (Mg, Mr, Mo,Msl); G′c, H (Msy)
β-Tubulin geneP (Mr, Mf,Msy, Msl, Mp,Mo)d; N (Mg,Mf, Msy, Msl,Mp)e
  • a Mf, M. furfur;Msl, M. slooffiae; Msy, M. sympodialis; Mp, M. pachydermatis;Mo, M. obtusa; Mg, M. globosa; Mr, M. restricta.

  • b Only 3 of 37 M. sympodialisstrains had this PCR type (see Fig. 2B).

  • c Only 2 of 37 M. sympodialisstrains had this PCR type. Restriction with NcoI produced two bands of 500 and 200 bp (PCR-REA type G′) instead of one 350-bp band of double intensity (PCR-REA type H).

  • d Positive amplification with primers for β-tubulin gene.

  • e Negative amplification with primers for β-tubulin gene.