Table 1.

Sources, origins, and multilocus genotypes of 78 strains from seven Malassezia species

MalasseziaspeciesStrainaOriginComplex PCR-REA typed
M. furfur (n = 13)CBS 1878, NTb DandruffA′CFN
JF 04SwedenACFP
GM 551London, United KingdomACFN
97 FR-1272Blood culture, PHLOg ACFP
97 FR-3007f Bronchial wash, PHLOACFN
97 F-661f Toenail swab, PHLOACFN
98 F-3617Neck, PHLOACF–
97 F-8817Left arm, PHLOAC–P
98 F-5399f Trunk, PHLOACFP
98 F-10017Right index nail, PHLOACFN
99 F-542PHLOA′CFN
99 FR-178PHLOACFN
M. furfure 99 F-1436PHLOACFN
M. globosa (n = 10)98 F-3552Anticubital area, PHLOBDG–
98 F-4888Back, PHLOBDGN
98 F-6443Body, PHLOBDG–
98 F-7317f Chest, PHLOBDGN
98 F-8304f Scalp hair, CanadaBDG–
99 F-160PHLOBD–N
YKM 48Forehead, PHLOB–GN
YKM 58Forehead, PHLOBD–N
YKM 45Forehead, PHLOBD–N
SF7Arm, South AfricaBDGN
M. obtusa (n = 3)98 F-3529Neck, CanadaBCGP
M. obtusae 98 F-8316Toenail, PHLOACGP
M. obtusae WF 7Trunk, HawaiiB–GN
M. pachydermatis (n = 3)ATCC 14521BCF–
CBS 1879, NTDog with otitis externa, SwedenBCFN
M. pachydermatise GM 420London, United KingdomACFP
M. restricta ( n = 5)CBS 7877, Tc Skin, United KingdomBDGP
YKM 8Forehead, PHLOBDGP
YKM 31Scalp, PHLOBDGP
YKM 32Scalp, PHLOBDGP
YKM 53Scalp, PHLOBDGP
M. slooffiae (n = 7)CBS 7956, THealthy ear of pigAC′GP
JF 06SwedenAC′GN
TV1f Skin, PHLOA′–GN
98 F-4721f Scalp, PHLOAC′GP
98 F-5360Neck, PHLOAC′GP
98 F-6419Groin, PHLOA–GN
99 F-411PHLOA′C′–N
M. sympodialis (n = 37)CBS 7222, THuman ear, United StatesAEHN
GM 323London, United KingdomAD′–N
97FR-2125Skin from chest, PHLOAEHN
97 F-8615f Upper chest, PHLOAEHN
98 F-4202Trunk, PHLOAEHN
98 F-4769Back, PHLOAEHP
98 F-4784Trunk, PHLOAEHP
98 F-4941Abdomen, PHLOAEG′P
98 F-5394Back, PHLOAEHN
98 F-5413Back, PHLOAEHN
98 F-5763Neck/back, PHLOAEHP
98 F-5850f Left breast, PHLOAEHP
98 F-6388Body, PHLOAEHN
98 F-7582Chest, PHLOAEHN
98 F-7807PHLOAEHN
98 F-8046Trunk, PHLOAEHN
98 F-8139Upper back, PHLOAEHN
98 F-9662Skin of scalp, PHLOAEHN
98 F-9846Back, PHLOAE-N
98 F-9925Trunk, PHLOAEHN
98 F-10007Back, PHLOAEHN
98 F-10770PHLOAEHN
98 F-10143PHLOAEHN
98 SF-4645CVP line swab, PHLOAEHP
99 F-440PHLOAEG′N
99 F-869PHLOAD′–P
99 F-902PHLOAEHN
99 F-944PHLOAEHN
99 F-946PHLOAE-N
99 F-1072PHLOAEHN
99 F-1181PHLOAEHN
99 F-1192PHLOAEHN
99 F-1407PHLOAEHN
YKM 56London, Ontario, CanadaAEHN
YKM 73London, Ontario, CanadaAD′HP
SF4Back, South AfricaAEHN
  • a CBS, strains bought from the Centraalbureau voor Schimmelcultures; ATCC, strains bought from the American Type Culture Collection; GM, strains obtained as a gift from Gillian Midgley, Department of Medical Mycology, St. John's Institute of Dermatology, London, United Kingdom; JM, strains obtained as a gift from Jan Faergemann, Department of Dermatology and Venereology, Göteberg University, Göteberg, Sweden; 97/98/99 F, 97/98/99 FR, and 97/98/99 SF, strains that were sent for mycological analysis to the Medical Mycology Laboratory, Public Health Laboratories of Ontario, Etobicoke, Ontario, Canada; YKM, strains isolated by Yatika Kohli from contact plates; SF, strains isolated from specimens obtained from South Africa; WF, strains isolated from specimens obtained from Hawaii.

  • b NT, neotype for Pityrosporum ovale(CBS 1878) or Pityrosporum pachydermatis (CBS 1879).

  • c T, ex-type isolate.

  • d The multilocus genotype represents the alleles observed for each of the strains analyzed by PCR-RFLP. The first letter designates one of the three alleles observed at the LSU locus with the restriction enzyme AvaI. The second letter designates one of the five alleles observed at the ITS locus with the restriction enzyme EcoRI. The third letter designates one of the four alleles observed at the ITS locus with restriction enzymeNcoI. The fourth letter designates the presence (P) or absence (N) of a fragment amplified after hot start-TD PCR with primers for the β-tubulin gene. –, missing data.

  • e Some ambiguity remains regarding the correct identification of this strain (see Results).

  • f Strain accessioned in the Centraalbureau voor Schimmelcultures yeast culture collection.

  • g PHLO, Public Health Laboratories of Ontario, Etobicoke, Ontario, Canada.