Table 2.

MseI restriction pattern of therrf-rrl spacer of B. burgdorferi sensu lato amplified by nested PCR directly from ticks or cultured strains

Strain or tick-derived sampleaAmplicon size (bp)RFLP patternMseI restriction fragment size (bp)b
B. burgdorferiZS7227A95, 37, 29, 28
B. afzelii ACA 1220B20, 37, 68, 95
B. garinii ZQ 1227C95, 95, 37
B. valaisiana UK229D162, 37, 23, 7
B. lusitaniae PotiB1230E95, 67, 39, 29
GT058c(I)d230E95, 67, 39, 29
GT078 (V)230E96, 66, 39, 29
GT151 (VI)231E96, 67, 39, 29
GT172 (VII)230E95, 67, 39, 29
GT132 (IV)229F95, 66, 52, 16
GT098 (II)212G95, 78, 39
GT167 (VIII)212G95, 78, 39
GT156 (III)220H95, 66, 30, 29
GT158 (IX)213G+E96, 78, 39
GT163 (X)230F+E95, 67, 29, 39
  • a Only representative samples are shown.

  • b The exact sizes of the fragments were determined from the sequence of the nested PCR product.

  • c Sequence identical to that of PotiB1.

  • d Roman numerals in parentheses indicate sequence type.