Table 6.

Sensitivities and specificities of all assays evaluated

Specimen typeAssay evaluated and resultNo. of specimensSensitivity (%)Specificity (%)
Culture positiveCulture negative
BALLegionella genus LC-PCRa
 Positive90100100
 Negative010
DFA (genus pool)
 Positive3033100
 Negative610
L. pneumophilaLC-PCRb
 Positive70100100
 Negative012
Open lung biopsyLegionella genus LC-PCRa
 Positive11069100
 Negative58
DFA (genus pool)
 Positive7044100
 Negative98
WS staining
 Positive10063100
 Negative68
L. pneumophilaLC-PCRb
 Positive2017100
 Negative128
L. pneumophilaISHc
 Positive140100100
 Negative010
  • a Assay target is 5S rDNA and is genus specific only.

  • b Assay target is mip gene and isL. pneumophila species specific.

  • c Assay target is 16S rDNA and is L. pneumophila species specific.