Table 1.

Tm of p66 andrecA gene fragmentsa

Species (strain) or patient no.DiagnosisTm(°C) calculatedMean Tm (± SD) (°C)
p66 gene ampliconrecA gene amplicon
B. burgdorferi sensu stricto (B31)81.778.72 ± 0.2884.67 ± 0.28
B. afzelii (NE 632)79.077.27 ± 0.3384.20 ± 0.40
B. garinii79.077.24 ± 0.3782.89 ± 0.49
1EM77.6584.24
2EM77.3784.26
3EM77.5784.62
4EM77.6384.26
5ACA77.95NAb
6EM76.87NA
7EM77.15NA
8EM79.23NA
  • a Tms of thep66 gene fragment were calculated by the oligoapplet program and obtained by LightCycler PCR analysis of the p66 gene amplicon and the recA gene amplicon of the three reference strains of B. burgdorferi sensu lato and of patients with EM or ACA. For the three reference strains, meanTms ± SD for 10 independent experiments are given.

  • b NA, no amplification of BorreliaDNA could be detected.