Table 3.

Nucleic acid targets for primers and probes

AssayPurposeTargetbProduct sizePrimer nameSequence and label(s) (5′→3′)d
ISHProbeL. pneumophila 16SM36026aP1aATC TGA CCG TCC CAG GTT
ISHProbeL. pneumophila 16SM36026P2AGC TTT CAT CCA AAG ATA
PCRPrimerL. pneumophila mipS7244224LPmipAfACC GAA CAG CAA ATG AAA GA
PCRPrimerL. pneumophila mipS72442124LPmipArAAC GCC TGG CTT GTT TTT GT
PCRPrimerLegionella species 5SX05081105L5FcACT ATA GCG ATT TGG AAC C
PCRProbeLegionella species 5SX05081L5PcRCAT GAG GAA GCC TCA CAC TAT CAP
PCRPrimerLegionella species 5SX05081105L5RBcGGC GAT GAC CTA CTFT TC
PCRProbeL. pneumophila mipS72442LPP1AAC AAG TTT CAG AAA GAT TTG ATG GCA AAGF
PCRProbeL. pneumophila mipS72442LPP2RGTA CTG CTG AAT TCA ATA AGT AAG CGG ATGP
  • a Previously described, as LEGPNE1, by Grimm et al. (19).

  • b GenBank accession numbers are given.

  • c Modified from sequences described by Mahbubani et al. (35).

  • d R, LC-Red 640 fluorescent dye; F, LC fluorescein dye; P, phosphate.