Table 3.

Oligonucleotides used for real-time PCR assay

Primer, product, or probe (sequence)Virus and positions amplifiedaLength (nucleotides)G+C content (%)Melting temp (°C)
Primers
 Forward (5′-CATCACCGACCCGGAGAGGGAC)HSV-1, 65866–65887; HSV-2; 66339–663602268.266.9
 Reverse (5′-GGGCCAGGCGCTTGTTGGTGTA)HSV-1, 65936–65957; HSV-2, 66409–664302263.669.0
ProductHSV-1, 65866–65957; HSV-2, 66430–6633992
TaqMan probe (5′-6FAM-CCGCCGAACTGAGCAGACACCCGCGC-TAMRA)HSV-1, 65907–65932; HSV-2, 66380–664052673.179.8
  • a GenBank accession numbers: X14112 (HSV type 1 complete genome) and Z86099 (HSV type 2 complete genome).