Table 2.

Oligonucleotides used for home-brew molecular assay

Assay step and primer (sequence) or productVirus and positions amplifiedaLength (nucleotides)G+C content (%)Melting temp (°C)
Initial PCR
 Primers
  Forward (5′-TGCTCCTACAACAAGTC)HSV-1, 138845–138861; HSV-2, 141422–1414381747.143.8
  Reverse (5′-CGGTGCTCCAGGATAAA)HSV-1, 139028–139044; HSV-2, 141625–1416411752.951.4
 ProductHSV-1, 138845–139044; HSV-2, 141422–141641200
Nested PCR
 Primers
  Forward (5′-ATCCGAACGCAGCCCCGCTG)HSV-1, 138878–138897; HSV-2, 141475–1414942070.070.1
  Reverse (5′-TCTCCGTCCAGTCGTTTATCTTC)HSV-1, 138997–139019; HSV-2, 141594–1416162347.857.3
 ProductHSV-1, 138878–139019; HSV-2, 141475–141616142
  • a GenBank accession numbers: X14112 (HSV type 1 complete genome) and Z86099 (HSV type 2 complete genome).