Table 2.

Reactivity of colostra to EPEC antigens by ELISA and immunoblottinga

Sample no.BfpAEspAEspB
EIbEIbEIb
116+16+
2++16+
340++
480++
5320++
680+256+8+
7
8160+8+
980+16+16+
1040256+
11320+16+
12+
13160+128+
1432+
15+256+128+
1632+32+
17+
18256+64+
1940+12832+
2016+
2116+8+
  • a The numbers in columns E (ELISA) represent the titer of each sample expressed as the reciprocal of the dilution at which a positive reaction was observed (for BfpA, 1:10 to 1:20 = negative, 1:40 to 1:80 = weak positive, and >1:160 = positive; for EspA and EspB, 1:2 to 1:4 = negative, 1:8 to 1:16 = weak positive, and >1:32 = positive). Dilutions with absorbance values below 0.2 were considered negative. Ib, immunoblotting.