Table 1.

Digestion time, incubation temperature, and discriminatory ability of 15 restriction enzymes used for characterization of 16 L. monocytogenes isolates

EnzymeIncubation temp (°C)Digestion time (min)No. of ribotypes among 16 isolatesSID
PvuII3720150.992
EcoRI3720120.950
BstEII60120100.925
BanI37120100.917
XhoI37120100.900
BglII3712080.883
EagI3712090.883
AseI3712070.817
HincII3712050.792
PstI3712060.617
NcoI3712040.617
XbaI3712010
BsoBI60120NDaND
ClaI37120NDND
StyI37120NDND
  • a ND, not determined. The enzymesBsoBI, ClaI, and StyI showed incomplete digests with spurious and variable high-molecular-weight bands, which did not allow assignment of distinct ribotypes.