Table 1.

Primer sequences for amplification and sequencing of theflaA gene of B. clarridgeiaeand B. bacilliformis

Primer namePrimer sequenceSpecificity
prCHD1265′-GATTTCTAGATTTAAGAAGGAGATATACATATGGGT TCTAGTATATTAACTAATAGA-3′Forward, positions 180–206 of B. bacilliformis flaA (accession no.L20677); additional sequences including XbaI and ribosome binding site at 5′ (highlighted in boldface)
prCHD1275′-CGGGATCCCTGTAAATGCAGCATGTCCCTT-3′Forward, positions 130–151 of B. bacilliformis flaA (accession no.L20677); additional sequences including EcoRI site at 5′ (highlighted in boldface)
prCHD1285′-CCCAAGCTTACGGAACAAACCTAAAATATTCTG-3′Reverse, positions 1329–1306 of B. bacilliformis flaA (accession no.L20677); additional sequences including HindIII site at 5′ (highlighted in boldface)
prCHD1515′-AATATCCTTTCCAATGGTGGT-3′Forward, positions 548–568 of B. bacilliformis flaA (accession no.L20677);
prCHD1535′-CAGCAACAGCTTGGTATTCAGGCTC-3′Forward, positions 1152–1176 of B. clarridgeiae flaA (this work)
prCHD1655′-CAAATAAGGGAGAACAACATTGTA-3′Reverse, 136–113 bp downstream of B. clarridgeiae flaA (this work)
prCHD1685′-CTTGGCGAGTTACATTCAGAC-3′Forward, positions 915–935 of B. clarridgeiae flaA (this work)
prCHD1695′-AGTCTGACCTCCATTGGAAAG-3′Reverse, positions 455–435 of B. clarridgeiae flaA (this work)
M13-forward5′-TGTAAAACGACGGCCAGT-3′
M13-reverse5′-CAGGAAACAGCTATGACC-3′