Table 4.

Oligonucleotide primers used for PCR amplification and sequencing to determine the gltA sequences of various species and to confirm sequences of the gltA coding region in other ehrlichial strains studieda

Target speciesPrimerSequence (5′-3′)c
HGE agent, E. equi, E. phagocytophila HG-M28FGTA-ATA-AAT-TGT-ATT-ATC-AGA-G
HG1257RAAT-ACG-TGA-GTT-TGA-AAC-CA
HG602Fb TGG-ATG-ATG-CAC-ATC-GTG
HG700Ra TAC-GCA-CAG-TGG-AAG-TAG
A. marginale Florida and South IdahoMAR-M35FGTC-TGG-TGA-GTT-TGT-TGT-CC
MAR-1287RGCT-TGC-ACA-TCG-CTC-AAT-AA
A. centrale CENT-M32FGTG-TCC-AGT-AAA-CTT-GTT-GTC-GG
CENT1534RAAA-GCA-TGG-TGC-GAG-CAT-A
Ehrlichia detected from I. ovatus CAN-M61FTTA-TCT-GTT-TAT-GTT-ATA-TAA-GC
R1bCGA-TGA-CCA-AAA-CCC-AT
F1bGAT-CAT-GAR-CAR-AAT-GCT-TC
MUR1251RCTA-GAT-TTT-TGT-AAT-ATG-GCC-AG
E. chaffeennsis CAN-M61FTTA-TCT-GTT-TAT-GTT-ATA-TAA-GC
CHAFF1285RAAA-CAA-TAA-GCA-ATG-ATA-ATT-CAA
E. muris CAN-M61FTTA-TCT-GTT-TAT-GTT-ATA-TAA-GC
MUR1278RAAT-TTG-ATA-ACA-ATA-GCA-TAA-AAA-C
E. canis CAN-M61FTTA-TCT-GTT-TAT-GTT-ATA-TAA-GC
CAN1317RCAG-TAC-CTA-TGC-ATA-TCA-ATC-C
C. ruminantium CR-M31FACG-CTT-TGT-TGT-TAT-TGT-ATT-AG
CR1278RCTA-CAA-AAG-GAA-ATA-CCT-TCA-C
E. sennetsu, E. risticii SEN-M26FCTC-AAC-TGG-AGA-ATA-TTT-AAA-GAA-G
SEN–RIS-1218RGCG-KAG-AAC-CAC-TAA-MAG-CTG
N. helminthoeca NH-M91FCAT-AGA-TTA-ACT-GTG-CTA-C
NH1239RGAT-GAA-ATT-CCA-TCC-TCG-TGT-G
  • a Specific or degenerated primers shown in Table 3 were also used as supplementary sequence primers if necessary.

  • b Sequence primers.

  • c  R, A or G; M, A or C.