Table 2.

Oligonucleotide primers used for PCR to amplify partial citrate synthase gene

PrimerSequence (5′-3′)aOrganisms in which primer is used
Forward
 F3TCT-TCT-CAT-CCT-ATG-GCHGE agent
 F4bCCG-GGT-TTT-ATG-TCT-ACT-GCHGE agent, E. equi, E. phagocytophila, A. marginale South Idaho, A. marginale Florida, E. chaffeensis, E. muris, C. ruminantium
 F4eACT-GCT-TCK-TGT-SAR-TC N. helminthoeca
 F1CAT-GAR-CAR-AAT-GCT-TC E. sennetsu
 Chaf-FGGA-TTA-TGG-TWR-AAR-AAG-C E. canis
 EH130FGGW-TTY-ATG-TCY-ACT-GCT-GC A. centrale
 SEN330FAAA-TAT-CCG-TTC-TTT-CCC-AAC-G E. risticii
Reverse
 R1bCGA-TGA-CCA-AAA-CCC-ATHGE agent, E. equi, E. phagocytophila, A. marginale South Idaho,A. marginale Florida, E. chaffeensis, E. muris, C. ruminantium, N. helminthoeca, E. sennetsu
 Chaf-RTAY-AAC-TGR-CGT-GGR-CG E. canis
 HG1085RACT-ATA-CCK-GAG-TAA-AAG-TC A. centrale
 SEN890RGCT-TTA-ATA-TGG-CTG-CAC-GAG E. risticii
  • a  S, G or C; R, A or G; W, A or T; Y, C or T; K, G or T.