Table 1.

Characteristics of SF STEC O157:H strains isolated from a cow and patients and of control NSF STEC O157:H7 strain EDL 933

StrainaDisease; countryb (referencec)Phenotypic characteristicsdChromosomal characteristicsPlasmid-encoded genesg
SerotypePTSF/GUDStxEHEC Hlystxe eaeP-gene profilefEHEChlykatPespPetp
258/98h HUS; CRO157:H 88+/+2 stx 2/18.0γ17−/−−/−−/−−/−
269/98h WD; CRO157:H 88+/+2 stx 2/18.0γ17−/−−/−−/−−/−
550/98h Cow; CRO157:H 88+/+2 stx 2/18.0γ17−/−−/−−/−−/−
703/88HUS; G (10)O157:H 88+/+2 stx 2/18.0γ17+/48.5−/−−/−+/3.9, 1.9i
221/95HUS; CR (2)O157:H 88+/+2 stx 2/18.0γ9+/15.0−/−−/−+/3.7, 1.7
1995/96HUS; G (2)O157:H 88+/+2 stx 2/18.0γ16+/15.0−/−−/−+/3.9, 1.9
3573/98HUS; GO157:H 88+/+2 stx 2/18.0γ16+/15.0−/−−/−+/3.9, 1.9
EDL 933HC; US (15)O157:H721−/−1 and 2+ stx 1/NPγ2+/12.0+/9.0+/7.5+/3.9, 1.9
stx 2/4.7
  • a For the SF STEC O157:Hstrains, the last two numbers indicate the year of isolation.

  • b WD, watery diarrhea; HC, hemorrhagic colitis; CR, Czech Republic; G, Germany; US, United States.

  • c Strains for which no references are given are from this study.

  • d PT, phage type; SF/GUD, sorbitol fermentation/β-d-glucuronidase activity; Stx, Stx phenotype; EHEC Hly, production of EHEC hemolysin; +, positive result; −, negative result.

  • e stx PCR result/size (in kilobases) of EcoRI restriction fragment of the genomic DNA hybridizing to the stx 2 probe. NP, not performed.

  • f P-gene profile 17 is characterized by three fragments of 20, 6.4, and 3.7 kb; the relatedP-gene profile 16 is characterized by two fragments of 20 and 3.7 kb, and the other related P-gene profile 9 is characterized by two fragments of 6.4 and 3.7 kb. P-gene profile 2 consists of two fragments of 21.2 and 16.2 kb and is unrelated to P-gene profiles 17, 16, and 9.

  • g Detection of the gene by PCR/size (in kilobases) of the plasmid DNA fragment hybridizing to the respective probe. +, positive result; −, no signal obtained.

  • h Epidemiologically related strains; the other four SF STEC O157:H strains are representative isolates from 1988 to 1998 and are epidemiologically unrelated.

  • i Fragments of two different sizes hybridized with the probe.