Table 1.

B. cepacia complex strains used to develop molecular diagnostic approaches

Genomovar and straina16S rDNADdeI RFLPbResult ofrecA RFLP by digestion with:recA GenBank accession no.recA phylogenetic and PCR identification group
HaeIIIcMnlId
B. cepacia genomovar I
 ATCC 25416T 2Dd AF143786
 J1050e 2DdI
 ATCC 177592Ee AF143788 I
 CEP5092Ee AF143787 I
 LMG 179972EeI
B. multivorans(formerly genomovar II)
 C53933Fa AF143776 B. multivorans
 LMG 13010T 3Fa B. multivorans
 CF-A1-13Fa B. multivorans
 C15763Ca AF143774 B. multivorans
 HI-2308e 3Ca AF143777 B. multivorans
 JTC3Fa AF143778 B. multivorans
 C19623Fa B. multivorans
 ATCC 176163Fa AF143775 B. multivorans
 249-23Fa B. multivorans
B. cepacia genomovar III
 J23152GfIII-A
 BC72GfIII-A
 K56-22Gf AF143779 III-A
 C54242Gf AF143781 III-A
 C64332Gf AF143780 III-A
 C4455e 2Gf AF143782 III-A
 C13942Hg AF143783 III-B
 ATCC 177652HgIII-B
 CEP5112Ih AF143785 III-B
 PC1842Ji AF143784 III-B
B. stabilis (formerly genomovar IV)
 LMG 07000e 2Jb AF143789 B. stabilis
 LMG 14291e 2Jb AF143790 B. stabilis
 LMG 142942Jb B. stabilis
 C73222Jb B. stabilis
 LMG 140862Jb B. stabilis
 LMG 188882Jb B. stabilis
B. vietnamiensis (formerly genomovar V)
 PC2591Ac AF143791 B. vietnamiensis
 LMG 162321Ac B. vietnamiensis
 FC4411Ac B. vietnamiensis
 LMG 10929T 1Bc AF143793 B. vietnamiensis
 C2822e 1Bc AF143792 B. vietnamiensis
  • a All strains except those indicated in footnote b below were derived from the B. cepaciacomplex strain panel where source and other data relevant to each are described (18).

  • b Data are numerical 16S rRNA RFLP types as indicated in Fig. 1.

  • c Letters correspond to alphabetical RFLP types as shown in Fig. 2A.

  • d Letters correspond to alphabetical RFLP types as shown in Fig. 2B.

  • e Strains not derived from the B. cepacia complex panel (18): J1050, isolated from non-CF patient within the U.K. and kindly provided by J. R. W. Govan; HI-2308, recovered from neonatal infection and kindly provided by J. J. LiPuma; C4455, recovered from a patient with CF (16); LMG 07000, recovered from a patient with septicaemia (20); LMG 14291, recovered from a patient with CF (20); and C2822, recovered from a patient with CF (16).