Table 3.

Cross-reactivity in ELISA betweenRhodotorula mannan and sera of leptospirosisa

SampleBlood-collecting days after onset of diseaseSerogroupDetection (avg absorbance)
IgMIgG
JM-1f25Autumnalis+ (0.461)+ (0.420)b
s49− (0.103)+ (0.419)
JM-2f10Autumnalis− (0.048)+ (0.401)
s114− (0.096)+ (0.552)c
JM-3f5Icterohaemorrhagiae− (0.052)+ (0.240)
s20+ (0.220)+ (0.461)
JM-4f6Icterohaemorrhagiae− (0.100)+ (0.185)
s24+ (0.148)+ (0.444)
JM-5f10Icterohaemorrhagiae+ (0.407)c+ (0.426)b
s17+ (0.495)d+ (0.494)c
JM-6f3Icterohaemorrhagiae+ (0.074)+ (0.167)
s17− (0.096)+ (0.162)
JM-7f8Icterohaemorrhagiae+ (0.351)d+ (1.178)d
s36+ (0.387)c+ (0.920)d
JM-8f11Icterohaemorrhagiae+ (0.358)d+ (0.989)d
s24− (0.080)+ (0.442)
JM-9f21Icterohaemorrhagiae− (0.019)− (0.057)
s65− (0.031)− (0.070)
JM-10f43Unknowne+ (0.250)+ (0.353)b
s149+ (0.193)+ (0.391)b
JM-11f7Unknowne+ (0.171)+ (0.174)
s17+ (0.199)+ (0.172)
JM-12f9Unknowne+ (0.262)+ (0.220)
s36+ (0.384)+ (0.307)
JM-13f30Unknowne+ (0.266)+ (0.390)
s37+ (0.244)+ (0.392)
JM-14f3Unknowne− (0.101)+ (0.126)
s15+ (0.129)+ (0.149)
JM-15f30Unknowne+ (0.311)+ (0.337)b
s74+ (0.147)+ (0.338)
  • a Unless otherwise indicated, triplicate assays were done by using each 500-fold-dilution serum, and the average data are shown in parentheses. +, positive samples giving an average absorbance higher than twice the control value (0.045).

  • b–d Assayed with 1,000-, 2,000-, or 5,000-fold-dilution sera, respectively.

  • e Serum which did not agglutinate the respective cells of serovars copenhageni, autumnalis, hebdomadis, and australis in MAT procedure.