Table 2.

Comparison of the NucliSens isolation kit sensitivity to other extraction methods from spiked specimens as determined by mean time-resolved fluorescence valuesa

Specimen (concn [PFU/ml])Mean time-resolved fluorescence values (P value) for paired tests:
NS/PGNS/QIANS/MPNS/IQNS/GCC
BAL (4)293,768/242,640 (0.3807b)293,768/123,226 (0.0255)293,768/375,405 (0.3637b)293,768/97,325 (0.0654b)293,768/14,300 (0.0009)
BLD (4)292,375/79,808 (0.0265)292,375/587,679 (0.5139b)292,375/42,176 (0.0235)292,375/63,402 (0.0087)292,375/220,722 (0.4887b)
Plasma (4)359,998/296,304 (0.6317b)359,998/123,173 (0.0105)359,998/303,136 (0.3558b)359,998/47,882 (0.0022)359,998/8,894 (0.0027)
CSF
 4662,433/527,460 (0.4165b)662,433/65,469 (0.0121)662,433/97,573 (0.0416)662,433/31,787 (0.0085)662,433/1,486 (0.0076)
 272,042/64,069 (0.7741b)
 0.835,542/25,847 (0.4816b)
 0.428,943/8,193 (0.1353b)
  • a Six replicates were performed from each sample. Results are presented as pairwise comparisons and Pvalues (by paired t test; calculated with StatView).

  • b Not significant (P > 0.05).