Table 1.

Comparison of DNA extraction kit sensitivity from spiked specimens as determined by mean time-resolved fluorescence valuesa

Specimen (concn [PFU/ml])NSPGQIAMPIQGCC
Mean valueNo. posbMean valueNo. posbMean valueNo. posbMean valueNo. posbMean valueNo. posbMean valueNo. posb
BAL (4)293,7686/6242,6406/6123,2266/6375,4055/697,3254/614,3002/6
BLD (4)292,3756/679,8085/6587,6796/642,1764/663,4024/6220,7226/6
Plasma (4)359,9986/6296,3046/6123,1736/6303,1366/647,8822/68,8941/6
CSF
 4662,4336/6527,4605/665,4693/697,5732/631,7872/61,4860/6
 272,0425/664,0694/6
 0.835,5425/625,8473/6
 0.428,9433/68,1931/6
  • a Six replicates from each sample. pos, positive.

  • b Number of replicates out of six with a DELFIA fluorescence value of >15,000.