Table 2.

Specificity of oligonucleotide probes to DNA from yeast-like fungid

ProbeaMean EI ± SE (n) using DNA from:
H. capsulatumB. dermatitidisC. immitisP. brasiliensisP. marneffeiS. schenckiiC. neoformansbP. cariniiCandida speciesc
Dm11.0 ± 1.1 (34) 9.4 ± 1.4 (24)16.9 ± 2.2 (14)13.9 ± 1.1 (21)3.0 ± 0.5 (31)0000
Hc15.8 ± 1.4 (37)00000000
Bd011.9 ± 2.0 (20) 4.3 ± 0.8 (10)000000
Ci0021.9 ± 3.2 (13)000000
Pb00010.8 ± 0.8 (21)00000
Pm00007.6 ± 0.6 (36)0000
Ss0000023.6 ± 4.3 (12)000
Cn00000042.7 ± 2.7 (31)00
Pc00000006.2 ± 2.7 (10)0
  • a See Table 1 for definition of abbreviations.

  • b Includes DNA from serotypes A, B, C, and D.

  • c Candida species included C. albicans, C. glabrata, C. krusei, C. tropicalis, and C. parapsilosis; excludes results for Hc probe against C. albicans; mean EI ± SE = 6.5 ± 1.0 (n = 10) .

  • d Mean EI ± standard error (n) for all heterologous DNA tested with the following probes: Dm 1.13 ± 0.04 (n = 89) ; Hc, 1.2 ± 0.3 (n = 118) ; Bd, 1.41 ± 0.12 (n = 103) ; Ci, 1.0 ± 0.01 (n = 96) ; Pb, 1.01 ± 0.02 (n = 88) ; Pm, 0.99 ± 0.02 (n = 98) ; Ss, 0.99 ± 0.01 (n = 103) ; Cn, 0.98 ± 0.01 (n = 95) ; and Pc, 0.98 ± 0.01 (n = 96) . All probes significantly hybridized to homologous DNA but not heterologous DNA at P < 0.001 except for Pc (P < 0.05) and Bd versus C. immitis DNA (P < 0.01) . A value of 0 was assigned for all EI values less than 1.75 for ease of presentation.