Table 4.

Evaluation of the sensitivity of PCR-EIA

DNA concnaEIbReading in agarose gelc
10,00053.3+
2,00050.4+
40038.2+
8013.9+
165.3+
3.22.2
0.641.5
0.1281.2
0.02561.1
  • a Concentration is given in picograms per reaction.

  • b EI using H. capsulatum (Down's strain) DNA.

  • c +, visually positive band in gel after ethidium bromide staining.