Table 3.

PCR results from clinical matrices spiked with Ebola Zaire

No. of PFU/5 μl of templateaCTbvalue
PBSBloodSerumPlasma
15,62522.419.222.219.4
3,12522.722.221.723.1
62526.624.825.224.6
12529.428.626.926.5
2530.833.529.331.9
535.132.130.332.2
  • a Number of PFU present in the PCR mixture.

  • b CT is the cycle number at which the fluorescence generated within a reaction crosses the threshold. PBS, blood, serum, and plasma were spiked with known numbers of PFU of Ebola Zaire. The sensitivity of the EBOGP-1D primer-probe set was tested in these matrices.