Table 1.

Primers used in PCR reactions for flaAsequencing and expression of the respective protein

Target DNA Primer (5′-3′)aLocation (bp)
Sequencing
 1ATA TAA GGA GTT GGT TTA CAT−19–2
 2ATG AAA AGG AAA GCT AAA AGT1–21
 3GCT ATT CTC AAT CAT CTG C386–404
 4TTT ATG AGA CTA GCG GAA CT860–879
 5TTA AAT AAA CCT TGC CAT CAA1,438–1,418
 6TTT TCT AAA TCT AAT ATT TCC AT1,117–1,094
 7CAT TTT ACT TGA AGC AAG AG717–698
Expression
 FlaABbiaCCG GAT CCG ATG GAT TAG CAG AGG GTT67–85
CCG GTA CCC TAA TTT TTC GGA GAT GAT TC1,026–1,006
 FlaABaA91CCG GAT CCG ATG GAT TAA CAG AGG GC67–84
CCG GTA CCC TAA TTT TCT GAA GAT GAT TC1,026–1,006
 FlaABg46CCG GAT CCG ATG GAT TGC CAG AAG GC67–84
CCG GTA CCC TAC TTT TTT AAG GAT GAT TC1,029–1,009
  • a Underlining indicates BamHI and KpnI cleavage sites.