Table 1.

Primer and probe sequences used in the enterovirus real-time RT-PCR assay

Primer or probeSequenceTma(°C)Length (no. of nucleotides)
Forward primer5′-CCCTGAATGCGGCTAATCC-3′59.119
Reverse primer5′-ATTGTCACCATAAGCAGCCA-3′55.320
Probe5′-AACCGACTACTTTGGGTGTCCGTGTTTC-3′67.528
  • a Calculated by using Primer Express software version 1.0.