Table 1.

Number of HBV DNA PCR-positive samples following extraction by three different methods in EUROHEP and VQC standard dilution panels, using 100-μl sample input

Panel (geq/ml)No. positivea
Proteinase K + extractor10 min 80°C + extractorQ1Aamp extraction
EUROHEP
 40,000,0001/11/11/1
 40,0001/11/11/1
 4,0002/23/33/3
 1,2120/21/33/3
 4000/22/32/3
 1210/20/31/3
 400/20/30/2
 40/10/10/1
 0.40/10/10/1
VQC
 30,000,0001/11/11/1
 300,0001/11/11/1
 30,0001/11/11/1
 9,0902/23/33/3
 3,0001/23/33/3
 9090/22/33/3
 3000/22/32/3
 910/20/31/3
 300/10/10/1
 90/10/10/1
 30/10/10/1
 0.30/10/10/1
  • a Number positive/number tested.