Table 2.

Primers used for the detection and identification ofB. cepacia complex genomovars in CF sputum samples

PrimerSequence (5′ to 3′)Specificity
BCR1aTGA CCG CCG AGA AGA GCA ArecA gene—B. cepacia complex
BCR2aCTC TTC TTC GTC CAT CGC CTCrecA gene—B. cepacia complex
PSL1bAAC TAG TTG TTG GGG ATT CAT TTC16S rRNA—B. cepacia
PSR1bTTT CGA GCA CTC CCG CCT CTC AG16S rRNA—B. cepacia
G1cTCG GAA TCC TGC TGA GAG GC16S rRNA—genomovars I, III, and IV
G2cGCC ATG GAT ACT CCA AAA GGA23S rRNA—genomovars I, III, and IV
BC-GIIdAGG CGG TCT GTT AAG ACA16S rRNA—genomovar II
BC-GVdTAA TAC CGC ATA CGA TCT AT16S rRNA—genomovar V
BC-RdAGC ACT CCC GAA TCT CTT16S rRNA—genomovars II and V
PSLbAGG ATT AGA TAC CCT GGT AGT CCA16S rRNA—all bacteria
PSRbACT TAA CCC AAC ATC TCA CGA CAC16S rRNA—all bacteria
  • a Primers described by Mahenthiralingam et al. (23).

  • b Primers described by Campbell et al. (4).

  • c Primers described by Whitby et al. (34); also known as PC-SSF and PC-SSR (21).

  • d Primers described by LiPuma et al. (21).