Table 2.

Amplification parameters for Sa442 andmecA PCRs

Cycle parametersTemp (°C)Ramp rate (°C/s)Time(s)Fluorescence acquisitionNo. of cycles
Sa442mecASa442mecA
Denaturation9595201010None1
Amplification95952000None50
55572021None
7474321Single
Melt95952000None1
50502000None
95950.2Continuous