Table 2.

Interexperimental variability of RVFV RNA quantificationa

Sample no.No. of RVFV RNA copies/sample on day:No. of copies/sample (mean ± SD)CV (%)
123
13.5 × 1071.8 × 1073.2 × 1072.8 × 107 ± 0.200.9
21.2 × 1076.7 × 1061.4 × 1071.1 × 107 ± 0.130.55
31.6 × 1076.7 × 1061.5 × 1071.2 × 107 ± 0.261.1
47.7 × 1073.1 × 1074.8 × 1075.2 × 107 ± 0.663.0
5 (blank)Not detectedNot detectedNot detected
  • a Four identical samples from the supernatants of Vero cells infected with RVFV strain MP12 were analyzed in four separate experiments with a 1-day interval. Synthetic RNAs were included in each experiment to determine the RNA copy numbers. CV were calculated by using the CT values.