Table 3.

HBV DNA PCR reactivity in consecutive patient serum samples compared to serology

Sample no.Resulta
HBV viral load (pg/ml)HBsAga-HBs (IU/ml)QIAamp + PCRbExtractor + PCRc
Patient A
 11,427NTNTPosPos
 242PosNTPosPos
 33.5NTNTPosPos
 40.9PosNTPosPos
 5NegPosNTPosPos
 6NegPosNTNegPos
Patient B
 11NTPosNTPosPos
 12NTPos402NegPos
 13NTNeg>1,000NegNeg
 14NTNeg>1,000NegNeg
  • a Pos; positive; Neg, negative; NT, not tested.

  • b Qiagen extraction using 200-μl sample input.

  • c 10 min at 80°C extractor protocol using 1-ml sample input.