Table 1.

Sensitivity and intraexperimental variability of RVFV strain MP12 synthetic RNAa

No. of synthetic RNAb copiesDetection rate (%)CT (mean ± SD)CV (%)
5 × 10610017.79 ± 0.201.1
5 × 10510022.22 ± 0.311.4
5 × 10410025.98 ± 0.471.8
5 × 10310029.81 ± 0.060.2
5 × 10210032.70 ± 0.361.1
10210035.14 ± 0.260.7
5 × 1012537.25cNDd
  • a Standard deviations and CV were calculated by using the CT values.

  • b RNA was diluted with water, and four identical samples were assayed in the same experiment.

  • c Standard deviation was not determined.

  • d ND, not determined.