Table 3.

Results of PCR analysis of culture-positive CF sputum samplesa

SampleGrowth on MAST agarResult obtained by method indicatedGenomovar determined byrecA-based RFLP analysisResults of 16S or 16S-23S rDNA PCR with primer pair:
recA-based PCR with primers BCR1 and BCR2recA-based RFLP with:
HaeIIIbMnlIcPSL1-PSR1BC-GII–BC-RG1-G2dBC-GV–BC-R
M1++GfIII-A++
M2++GfIII-A++
M9++GfIII-A++
M16++GfIII-A++
M18++GfIII-A++
M20++FaB. multivorans+++
M32++GfIII-A++
M37++FaB. multivorans++
M48++IhIII-B++
M54++GfIII-A++
M55++HhIII-B++
M64++GfIII-A++
M69++IhIII-B++
M71e++
M84++GfIII-A++
M85++HhIII-B+
M87e++
M89++GfIII-A++
M94++GfIII-A++
  • a +, positive result; −, negative result.

  • b Letters correspond to alphabetical RFLP types as shown in Fig. 1A and Table 1.

  • c Letters correspond to alphabetical RFLP types as shown in Fig. 1B and Table 1.

  • d Primers also known as PC-SSF and PC-SSR (21).

  • e Isolates grown on MAST selective agar were identified as B. gladioli.