Table 3.

PCR-RFLP typing of B. burgdorferi directly in patient tissue and isolates cultured from these tissues

OutcomebRFLP type(s)aNo. of tissue-culture pairsc
TissueCulture
Identical115
223
332
1 and 21 and 21
Selectiond 1 and 335
1 and 215
1 and 222
2 and 321
2 and 331
Different121
12 and 31
1 and 232
31 and 21
321
  • a Based on RFLP pattern of digested nested-PCR product; see Table 1.

  • b Comparison of RFLP typing result by direct PCR in tissue and in cultured isolate from same tissue.

  • c Total of 31 tissue and culture pairs. This does not include 20 specimens reported in Table 2 which did not yield evaluable cultures (19 negative and 1 contaminated).

  • d RFLP type of cultured isolate is identical to only one of the types of the mixture observed directly in tissue.