Table 1.

PCR-RFLP typing of B. burgdorferi clinical isolates cultured from skin or blood of Lyme disease patients

RFLP type(s)Restriction patternaNo. of positive isolates/no. total (%)
SkinBlood
1H1, M146/183 (25.1)15/34 (44.1)
2H2, M270/183 (38.3)15/34 (44.1)
3H2, M355/183 (30.1)3/34 (8.8)
1 and 29/183 (4.9)1/34 (2.9)
1 and 31/183 (0.6)0/34 (0)
2 and 32/183 (1.1)0/34 (0)
  • a Restriction patterns are based on digestion of the 941-bp nested PCR product with restriction endonuclease HinfI (H1 and H2) or MseI (M1, M2, and M3).