Table 1.

Host ranges of IS2404 and IS2606 as determined by PCR

Organism (strain and/or source)aPresence of 492-bp IS2404 PCR productbPresence of 332-bp IS2606PCR productb
M. ulcerans (Chant strain; clinical isolate, Victoria, Australia)++
M. ulcerans (13822/70; clinical isolate, Queensland, Australia)++
M. ulcerans (5145; clinical isolate, Zaire, Africa)++
M. ulcerans (9673; clinical isolate, Benin, Africa)++
M. marinum (NCTC 2275)
M. kansasii (NCTC 10268)
M. tuberculosis (H37Rv)
M. avium (ATCC 25291)
M. simiae (ATCC 25295)
M. malmoense (NCTC 11298)
M. haemophilum (CAP strain E8)
M. szulgai (NCTC 10831)
M. gordonae (NCTC 10267)
M. nonchromogenicum (NCTC 10424)
M. terrae (NCTC 10856)
M. chelonae (ATCC 10269)
M. phlei (ATCC 11758)
M. agric
M. anthracenicumc
M. asiaticumc
M. aurumc
M. austroafricanumc
M. celatumc
M. chitaec
M. diernhoferic
M. duvallic
M. flavescensc
M. fortuitumc
M. gadiumc
M. gastric
M. gilvumc
M. haemophilumc
M. heidelbergensec
M. interjectumc
M. intermediumc
M. intracelluarec
M. lentiflavumc +d
M. neoaurumc
M. obuensec
M. parafinicumc
M. parafortuitumc
M. pulverisc
M. rhodesiaec
M. scrofulaceumc
M. shimoideic
M. thermoresistablec
M. tokaiensec
M. triplexc
M. xenopic
Aeromonas sobria(environmental isolate)
Campylobacter jejuni (NCTC 11351)
Citrobacter freundii (ATCC 8090)
Clostridum perfringens (environmental isolate)
Enterobacter aerogenes (ATCC 13048)
Enterobacter agglomerans (environmental isolate)
Enterobacter cloacae (environmental isolate)
Enterococcus faecalis (ATCC 19433)
E. coli (ATCC 11775)
Klebsiella pneumoniae (environmental isolate)
Legionella pneumophila (ATCC43111)
Proteus mirabilis (environmental isolate)
Pseudomonas aeruginosa (ATCC 10145)
Salmonella typhimurium (environmental isolate)
Shigella sonnei (environmental isolate)
Streptococcus bovis (environmental isolate)
V. cholerae(environmental isolate)
Yersinia enterocolitica(clinical isolate)
Cryptosporidium parvum(clinical isolate)
Giardia intestinalis (clinical isolate)
Homo sapiens
  • aM.” represents “Mycobacterium” throughout.

  • b +, present; −, absent.

  • c Reference strains from the culture collection of the Queensland Diagnostic and Reference Laboratory for Mycobacterial Diseases.

  • d PCR product identity confirmed as IS2606 by nucleotide sequencing. Organism identity was confirmed by partial 16S rDNA nucleotide sequencing.