Table 1.

B. burgdorferi chromosome copy number

SampleQuantification methodnaRatiob
Purified B. burgdorferiLimiting-dilution assay241.3 ± 0.41
recA PCR product as external standard30.22 ± 0.030
Limiting dilution assay in presence of 200 ng of joint DNA240.92 ± 0.26
Culture-spiked spleenB. burgdorferi DNA as external standard in presence of 200 ng of spleen DNA21.0 ± 0.12
  • a n , number of samples.

  • b Ratio, number of recA molecules per estimated number of bacteria in sample. The error values are standard deviations of replicate samples.