Table 1.

Detection of HHV-8 DNA in semen and controls

ExptNested PCR performedNo. of HHV-8 PCR-positive specimens among:Result for positive controls with the following no. of HHV-8 copies/10 μl of template (n = 1 each):
Semen from:Negative controls
HIV-positive MSMa (n = 7)Artificial-insemination donors (n = 30)Molt-3 cells (n = 3)Water (n = 4)1,000100101
A1Yes0110+++
A2Yes0000+++
B1No1500++
B2Yes21312+++
C1No0000+++
C2No0000+++
D1Yes0402++++
D2Yes0100++
D3Yes0302+++
EYes1b 100++
  • a MSM, men who have sex with men.

  • b n = 6 due to one insufficient specimen.