Table 1.

fimA type-specific or 16S rRNA-specific primers used in this study

Primer setSequence (5′ to 3′)Size (bp)a
Universal primers for positive controlAGA GTT TGA TCC TGG CTC AG3,480
GGC TAC CTT GTT ACG ACT T
P. gingivalis 16S rRNATGT AGA TGA CTG ATG GTG AAA ACC197
ACG TCA TCC CCA CCT TCC TC
Type IfimA CTG TGT GTT TAT GGC AAA CTT C392
AAC CCC GCT CCC TGT ATT CCG A
Type II fimA ACA ACT ATA CTT ATG ACA ATG G257
AAC CCC GCT CCC TGT ATT CCG A
Type III fimA ATT ACA CCT ACA CAG GTG AGG C247
AAC CCC GCT CCC TGT ATT CCG A
Type IVfimA CTA TTC AGG TGC TAT TAC CCA A251
AAC CCC GCT CCC TGT ATT CCG A
  • a Expected size of PCR product.