Table 2.

Primers selected for PCR

Target speciesPrimer designationaSequencebAnnealing temp (°C)
Eubacteria Eub-16-15′-AGR GTT YGA TYM TGG CTC AG-3′56
Eubacteria Eub-16-25′-ACC GCG GCT GCT GGC AC-3′60
Burkholderia spp. and Ralstoniaspp.BuRa-16-15′-AGC ACT TTT GTC CGG RAA-3′52
Burkholderia spp. and Ralstoniaspp.BuRa-16-25′-AAT CCC CAA CAA CTA GTT G-3′54
B. cepacia, B. multivorans, and B. vietnamiensis CeMuVi-16-2457 5′-CCG RCT GTA TTA GAG CCA-3′55
B. gladioli Gl-16-2457 5′-CGA AGG ATA TTA GCC CTC-3′54
B. cepacia Ce-16-21028 5′-AGC ACT CCC RCC TCT CAG-3′c 59
B. multivorans andB. vietnamiensis MuVi-16-21028 5′-AGC ACT CCC GAA TCT CTT-3′54
B. mallei and B. pseudomallei MaPs-16-2457 5′-CTC CGG GTA TTA GCC AGA-3′56
Ralstonia pickettii Pi-16-2461 5′-TCG ACC CCA GGT ATT AAC-3′54
B. vietnamiensis, B. mallei, and B. pseudomallei ViMaPs-23-15′-CCT TTG GGT CAT CCT GGA-3′56
B. cepacia and B. vietnamiensis CeVi-23-25′-TCC TAC CAT GCG TGC AA-3′52
B. mallei Ma-23-25′-CAC CGA AAC TAG CA-3′42
  • a 16, 16S rDNA; 23, 23S rDNA; 1, sense (forward) primer; and 2, antisense (backward) primer.

  • b Wobble bases: M = A or C; R = A or G; and Y = C or T.

  • c Partially congruent with primer PSR1 of Campbell et al. (11), i.e., 5′-TTTCGAGCACTCCCGCCTCTCAG-3′.