Table 2.

Primers selected for PCR

Target speciesPrimer designationaSequencebAnnealing temp (°C)
EubacteriaEub-16-15′-AGR GTT YGA TYM TGG CTC AG-3′56
EubacteriaEub-16-25′-ACC GCG GCT GCT GGC AC-3′60
Burkholderia spp. and Ralstoniaspp.BuRa-16-15′-AGC ACT TTT GTC CGG RAA-3′52
Burkholderia spp. and Ralstoniaspp.BuRa-16-25′-AAT CCC CAA CAA CTA GTT G-3′54
B. cepacia, B. multivorans, and B. vietnamiensisCeMuVi-16-24575′-CCG RCT GTA TTA GAG CCA-3′55
B. gladioliGl-16-24575′-CGA AGG ATA TTA GCC CTC-3′54
B. cepaciaCe-16-210285′-AGC ACT CCC RCC TCT CAG-3′c59
B. multivorans andB. vietnamiensisMuVi-16-210285′-AGC ACT CCC GAA TCT CTT-3′54
B. mallei and B. pseudomalleiMaPs-16-24575′-CTC CGG GTA TTA GCC AGA-3′56
Ralstonia pickettiiPi-16-24615′-TCG ACC CCA GGT ATT AAC-3′54
B. vietnamiensis, B. mallei, and B. pseudomalleiViMaPs-23-15′-CCT TTG GGT CAT CCT GGA-3′56
B. cepacia and B. vietnamiensisCeVi-23-25′-TCC TAC CAT GCG TGC AA-3′52
B. malleiMa-23-25′-CAC CGA AAC TAG CA-3′42
  • a 16, 16S rDNA; 23, 23S rDNA; 1, sense (forward) primer; and 2, antisense (backward) primer.

  • b Wobble bases: M = A or C; R = A or G; and Y = C or T.

  • c Partially congruent with primer PSR1 of Campbell et al. (11), i.e., 5′-TTTCGAGCACTCCCGCCTCTCAG-3′.