Table 1.

Results of conventional methods and PCR for diagnosis of LCL, ML, or MCL

MethodSkin biopsy specimen from patients with LCL (n = 184)Mucosal biopsy specimen from patients with ML (n = 24)Patients with MCLa (n = 8)Total
Skin biopsy specimenMucosal biopsy specimen
No. positive/no. tested% PositiveNo. positive/no. tested% PositiveNo. positive/no. tested% Positive No. positive/no. tested% PositiveNo. positive/no. tested% Positive
H&E staining65/175372/2395/7711/33373/20835
Touch preparation76/176430/604/7570/2080/19142
Cultureb 86/144603/13230/102/45091/16256
PCR178/1849717/24717/71006/6100208/22194
  • a For five patients, biopsy specimens from both mucosal and skin lesions were obtained.

  • b The average rate of culture contamination was 22% (a total of 47 of 209 cultures: 34 of 178 for patients with LCL, 11 of 24 for patients with ML, and 2 of 7 for patients with MCL).