Table 2.

Epidemiological data of MRSA from the pediatric Hospital Dona Estefânia, Lisbon, Portugal

Yr(s) and isolate no.WardAntibiogram result (resistant to)aMethicillin disc assay resultbSpectinomycin assay resultcClonal type (mecA::Tn554::PFGE)d
1992
 HDE1NAe AMS, Cft, Oxa, Pen29SII::NH::D1
 HDE2NAAMS, Cft, Oxa, Pen30SII::NH::D1
 HDE3NAAMS, Cft, Oxa, Pen31SII::NH::D1
 HDE4NAAMS, Cft, Oxa, Pen25RI::E::E
 HDE5NAAMS, Cft, Oxa, Pen27SII::NH::D3
 HDE6NAAMS, Cft, Oxa, Pen31SII::NH::D1
 HDE7NAAMS, Cft, Oxa, Pen, Ery, Cip, Cld, Te14RI::E::E
 HDE9NAAMS, Cft, Oxa, Pen33SII::NH::D1
 HDE11NAAMS, Cft, Oxa, Pen23SII::NH::D1
 HDE12NAAMS, Cft, Oxa, Pen, Ery, Cip, Cld, Te15RI::E::A5
 HDE14NAAMS, Cft, Oxa, Pen, Ery, Cip, Cld, Te19RI::E::A2
 HDE15NAAMS, Cft, Oxa, Pen30SII::NH::D3
1993–1994
 HDE63ICU neonatologyAMS, Cft, Oxa, Pen, Ery, Cip, Cld, Te18RI::E::A3
 HDE65Surgery 1AMS, Cft, Oxa, Pen31SII::NH::D2
 HDE66Medicine urgencyAMS, Cft, Oxa, Pen32SII::NH::D3
 HDE67NurseryAMS, Cft, Oxa, Pen30SII::NH::D3
 HDE68ICU neonatologyAMS, Cft, Oxa, Pen, Ery, Cip, Cld, Te16RI::E::A4
 HDE71Delivery roomAMS, Cft, Oxa, Pen31SII::NH::D3
 HDE72ICU neonatologyAMS, Cft, Oxa, Pen, Ery, Cip, Cld, Te13RI::E::A4
 HDE73ICU neonatologyAMS, Cft, Oxa, Pen, Ery, Cip, Cld, Te16RI::E::A4
 HDE75Surgery 1AMS, Cft, Oxa, Pen, Ery, Cip, Cld, Te14RI::J::A1
 HDE77Surgery 4AMS, Cft, Oxa, Pen29SII::NH::D3
 HDE83ICU pediatryAMS, Cft, Oxa, Pen, Ery, Cip, Cld, Te13RI::E::A4
 HDE84ICU neonatologyAMS, Cft, Oxa, Pen, Ery30SII::NH::D3
 HDE91Surgery 2AMS, Cft, Oxa, Pen29SII::NH::D1
 HDE281Pediatric surgeryAMS, Cft, Oxa, Pen29SII::NH::D6
 HDE333NurseryAMS, Cft, Oxa, Pen26SII::NH::D3
1995
 HDE163Delivery roomAMS, Cft, Oxa, Pen23SII::NH::D4
 HDE174PneumologyAMS, Cft, Oxa, Pen25SII::NH::D3
 HDE203Surgery 1AMS, Cft, Oxa, Pen, Ery, Cip, Cld, Te12RI::E::A6
 HDE218ICU neonatologyAMS, Cft, Oxa, Pen, Ery, Cip, Cld13RI::E::A7
 HDE220ICU pediatryAMS, Cft, Oxa, Pen, Ery, Cip, Cld, Te10RI::E::A8
 HDE221PediatryAMS, Cft, Oxa, Pen27SII::NH::D3
 HDE231Medicine urgencyAMS, Cft, Oxa, Pen25SII::NH::D3
 HDE232Surgery 2AMS, Cft, Oxa, Pen24SII::NH::D1
 HDE233Surgery 1AMS, Cft, Oxa, Pen, Ery, Cip, Cld, Te, SXT31SXII::χ::F
 HDE255Surgery 1AMS, Cft, Oxa, Pen24SII::NH::D5
 HDE266OtorrhinolaringologyAMS, Cft, Oxa, Pen, Ery, Cld27SII::NH::D6
 HDE318NurseryAMS, Cft, Oxa, Pen, Ery, Cld (i)24SII::NH::D3
1996
 HDE287PediatryAMS, Cft, Oxa, Pen, Cld (i)26SII::NH::D3
 HDE288PneumologyAMS, Cft, Oxa, Pen26SII::NH::D3
 HDE301Surgery 4AMS, Cft, Oxa, Pen, Ery, Cip, Cld16SI::NH::A3
 HDE309Surgery 1AMS, Cft, Oxa, Pen, Ery27SII::NH::D7
 HDE325Surgery 3AMS, Cft, Oxa, Pen27SXVII::NH::G
 HDE340Infectious diseasesAMS, Cft, Oxa, Pen, Ery, Cip, Cld, Te11RI::E::A9
 HDE362Surgery 2AMS, Cft, Oxa, Pen, Ery31SII::NH::D1
 HDE364ICU neonatologyAMS, Cft, Oxa, Pen, Ery, Cip, Cld, Te12RI::E::A7
 HDE386ICU neonatologyAMS, Cft, Oxa, Pen, Ery, Cip, Cld, Te9RI::E::A7
1997
 HDE366ICU neonatology 3AMS, Cft, Oxa, Pen, Ery, Cip, Cld, Te9RI::E::A7
 HDE371ICU pediatryAMS, Cft, Oxa, Pen, SXT26SXI::NH::H
 HDE372ICU pediatryAMS, Cft, Oxa, Pen, Ery, Cip, Te9RI::E::A7
 HDE373ICU neonatologyAMS, Cft, Oxa, Pen, Ery, Te(i)23SII::NH::D1
 HDE383OtorrhinolaringologyAMS, Cft, Oxa, Pen, Ery(i), Cld24SII::NH::D5
  • a Antibiogram results represent resistance to the antibiotics ampicillin-sulbactam (AMS), cephalothin (Cft), oxacillin (Oxa), penicillin G (Pen), erythromycin (Ery), ciprofloxacin (Cip), clindamycin (Cld), tetracycline (Te), trimethoprim-sulfamethoxazole (SXT), and vancomycin (V). (i), intermediate resistance.

  • b Results of the 1-mg methicillin disc assay. Numbers indicate inhibition halos in millimeters.

  • c Isolates were tested for their ability to grow in the presence of 500 μg of spectinomycin per ml. R, resistant (confluent growth); S, susceptible (no growth).

  • d Clonal types were defined on the basis ofClaI-mecA polymorphs,ClaI-Tn554 insertion patterns, andSmaI-PFGE profiles. NH, no homology with the Tn554 probe.

  • e NA, data not available.