Table 1.

Comparison of real-time PCR and nested PCR for detection of B. burgdorferi in different tissues from micea

Time p.i.MouseSpleenKidneyLN φdLNFoot φFoot
StrainNo.Real-timebNestedcReal-timeNestedReal-timeNestedReal-timeNestedReal-timeNestedReal-timeNested
1 hBALB/c100014,425+04,181+
200024,225+012,491+
C3H10006,186+012,592+
231008,586+0751+
3 hBALB/c100012,984+01,702+
20007,073+013,462+
C3H100011,476+016,306+
200023,830+012,265+
6 hBALB/c10002,568+01,958+
20002,645+02,890+
C3H100018,019+011,999+
20009,730+0ND+
12 hBALB/c1000817+04,890+
20601,051+04,634+
C3H1000979+0287+
20001,255+0ND+
24 hBALB/c1000161+0649+
20001,717+01,563+
C3H1000171+0415+
2000216+0642+
72 hBALB/c100000918+
20000+0613+
C3H10000+0880+
2000285+513+ND+
8 daysBALB/c1000153+025,000+
2000+131+27+19,298+
C3H10002,683+028,000+
200066,000+0146,067+
15 daysBALB/c1252+56+3,750+1,500+659+1,269+
20+12+5,172+209+297+7,423+
C3H167+58+15,000+5,556+343+6,667+
297+120+160,714+4,894+13,889+5,429+
55 daysBALB/c100+602+0+697+9,032+
200+0+203+1,308+8,000+
C3H100+2,194+1,037+2,200+5,278+
2019+2+1,778+159+8,644+
  • a BALB/c and C3H/HeJ (C3H) mice were infected with 105 spirochetes via the right hind footpad, and determinations by the real-time and nested PCRs were made at the indicated time points postinfection (p.i.).

  • b Values are numbers of spirochetes per 106 mouse cells, as determined by real-time PCR for individual mice.

  • c With the same samples used for the real-time PCR, a nested PCR for the flagellin gene was performed as described in Materials and Methods, and the resulting amplification products were analyzed by agarose gel electrophoresis. Symbols: +, visualization of a 290-bp fragment; −, no fragment of correct size.

  • d φ, from noninfected side.

  • e ND, not determined.