Table 2.

Length determination (in base pairs) of the PCR fragment containing the ITS2 of known fungal isolates

OrganismStrain(s)aConventional IDPCR fragment length (bp)
Yeasts
Candida albicansATCC 10231, SCC, HSC1159, HSC67442, HSC67630, HSC68392, HSC81363API 20C-AUX279
Candida kefyrATCC 4135, SCCAPI 20C-AUX372
Candida guilliermondiiCAP F1-95, SCCAPI 20C-AUX321
Candida tropicalisATCC 66029, SCC, HSC75317, HSC75418, HSC75422, HSC78308, HSC78408, HSC81682, HSC81701, HSC71273, HSC71278, HSC29977, HSC71277API 20C-AUX269
Candida kruseiATCC 6258, SCC, CT1, K1865, CA37, E175API 20C-AUX282
Candida glabrataATCC 90030, SCC, HSC17253, HSC20818, HSC20819API 20C-AUX360
Candida zeylanoidesCAP F18-93API 20C-AUX316
Candida lusitaniaeATCC 42720API 20C-AUX199
Candida parapsilosisATCC 22019, SCC, HSC5966, HSC21193API 20C-AUX251
Candida pelliculosaATCC 8168API 20C-AUX317
Candida pseudotropicalisATCC 66028API 20C-AUX372
Candida stellatoideaATCC 36232API 20C-AUX279
Trichosporon beigeliiATCC 28592API 20C-AUX295
HSC6088API20C-AUX298
Geotrichum candidumATCC 34614API 20C-AUX, mannitol192
Cryptococcus neoformansATCC 90112API 20C-AUX, urease, phenoloxidase315
Cryptococcus albidusATCC 10666API 20C-AUX350
Cryptococcus laurentiiATCC 18803API 20C-AUX306
Rhodotorula rubraATCC 9449, SCC HSC20116, HSC37777API 20C-AUX348
Blastoschizomyces capitatusATCC 10663API 20C-AUX248
Saccharomyces cerevisiaeATCC 9763API 20C-AUX363
Malassezia furfurATCC 14521API 20C-AUX494
Other opportunistic fungi
Aspergillus fumigatusCAP F6-91Morphology284
Aspergillus flavusATCC 10124Morphology288
Aspergillus nigerATCC 16404Morphology290
Aspergillus terreusATCC 28301Morphology292
Alternaria alternataCAP F13-93Morphology285
Penicilliumsp.SCCMorphology283
Acremonium strictumCAP F4-96Morphology294
Fusarium solaniCAP F15-95Morphology286
Paecilomyces sp.SCCMorphology287
Rhizopus sp.SCCMorphology319
Absidia corymbiferaATCC 66271Morphology410
Mucor sp.SCCMorphology312
Cunninghamella bertholletiaeATCC 42115Morphology347
Pseudallescheria boydiiATCC 58085Morphology321
Scedosporium prolificansSCCMorphology303
Phialophora verrucosaATCC 28181Morphology308
Phialophora richardsiaeATCC 26465Morphology276
Fonsecaea pedrosoiATCC 28174Morphology319
Cladosporium carrioniiATCC 32279Morphology303
Wangiella dermatitidisSCCMorphology334
Exophiala jeanselmeiATCC 10224Morphology311
Stemphylium ilicisATCC 18160Morphology280
Dimorphic fungi
Penicillium marneffeiATCC 18224Morphology280
Sporothrix schenckiiATCC 10212Morphology, conversion to yeast phase310
Blastomyces dermatitidisHSC patient isolateNucleic acid probe315
Histoplasma capsulatumHSC patient isolateNucleic acid probe299
Dermatophytes
Microsporum canisCAP F4-90Morphology321
Microsporum gypseumCAP F20-90Morphology321
Microsporum audouiniiATCC 42558Morphology318
Microsporum nanumSCCMorphology324
Microsporum gallinaeSCCMorphology325
Microsporum cookeiSCCMorphology314
Trichophyton schoenleiniiSCCMorphology, nutritional and temp (°C) requirements306
Trichophyton terrestreSCCMorphology, nutritional and temp (°C) requirements298
Trichophyton violaceumSCCMorphology315
Trichophyton mentagrophytesATCC 28185Morphology, urease, hair performation303
Trichophyton rubrumATCC 28188Morphology307
Trichophyton tonsuransATCC 28942Morphology and nutritional requirements302
Trichophyton verrucosumCAP F15-1994, SSCMorphology, nutritional and temp (°C) requirements307
Epidermophyton floccosumATCC 52066Morphology366
  • a SCC and HSC, stock culture collection and patient isolates, respectively, obtained from the Health Sciences Centre, Winnipeg, Manitoba, Canada.