Table 1.

Primers, fluorescence-labeled probes, and MgSO4 concentrations for TaqMan amplification

Dengue virus subtypeForward primer (concn)Reverse primer (concn)
15′ ATC CAT GCC CAT CAC CAA TG 3′ (30 pmol)5′ CAG GGA TCC ACA CCA CTG ATC 3′ (10 pmol)
25′ ACA AGT CGA ACA ACC TGG TCC AT 3′ (40 pmol)5′ GCC GCA CCA TTG GTC TTC TC 3′ (10 pmol)
35′ TGG CAA CAG GTC CCT TTC TG 3′ (30 pmol)5′ TGG CGT TGG ATG CTA GTC TAA GA 3′ (5 pmol)
45′ GCG TGG TGA AGC CCC TAG AT 3′ (30 pmol)5′ GAA CAA CTA GTG AGC GGC CAT C 3′ (10 pmol)
TaqMan probe (concn)Length of amplified DNA (bp)MgSO4 concn (mM)
5′ FAM AAC ATC TTC CCA ACT GGA TAC ATG GGT TTT GTC 3′TAMRAa (5 pmol)1622.5
5′ FAM TGG GAT TTC CTC CCA TGA TTC CAC TGG 3′TAMRAa (5 pmol)1773.5
5′ FAM AAG AAA GTT GGT TAG TTC CCT GCA GAC CCC A 3′TAMRA2294
5′ FAM TGG CAC TTC CCT CCT CTT CTT GAA CGA C 3′TAMRA1862.5
  • a Probe binds to complementary strand.