Table 2.

Reactivities of MAbs used to distinguish G types 1 to 4 and 9 by serotyping EIA with a panel of cell culture-adapted rotaviruses

Rotavirus strainG serotypeReciprocal titer of MAb F45:1 in serotyping EIA formataOD450with given MAb (MAb G serotype specificity)b:
F45:1 (9)RV-4:2 (1)RV-5:3 (2)RV-3:1 (3)ST-3:1 (4)60 (VP7)
116E9>1 × 107 1.283 0.1840.1360.1730.116 1.450
F459>1 × 107 2.768 0.2000.1530.1990.243 2.657
WI619>1 × 107 0.970 0.1790.1270.1720.215 1.462
AU329>1 × 107 0.463 0.1870.1240.1680.210 0.747
Hoso42 × 106 2.586 0.0400.0390.040 2.495 2.868
ST-342 × 104 0.1300.0380.0360.043 1.654 2.839
RV-415 × 103 0.127 2.491 0.0370.0390.039 2.791
RV-527 × 103 0.1330.040 0.362 0.0350.036 0.955
SA1135 × 103 0.1100.0410.038 2.680 0.045 2.977
TFR4155 × 103 0.1040.0380.0370.0410.220 0.879
  • a All rotavirus strains tested reacted with MAb F45:1. However, the dilution of MAb F45:1 used to screen samples in the serotyping EIA was higher than its titers with RV-4, RV-5, SA11, and TFR41, so these rotaviruses were not detected in the serotyping EIA with the single optimal dilution of MAb F45:1.

  • b Positive reactions in the serotyping EIA, in which MAbs were used at a single optimal dilution, are shown in boldface.