Table 3.

Comparison of MAb serotyping EIA and RT-PCR for determination of G9 types of cell culture-adapted human rotaviruses

G genotype determined by RT-PCRNo. of strains with the following G serotype determined by MAb EIAa:Total
99+39+43Nontypeable
913110318
9+3100001
2000022
3000101
Nonreactiveb 200002
Total16111524
  • a All samples that contained viral antigen typeable by EIA also reacted with MAb 60, whereas none of the samples that contained nontypeable antigen reacted with MAb 60.

  • b No RT-PCR product was visible, even though a product of the expected size from control prototype rotaviruses was obtained.