TABLE 3.

Species specificity of two-temperature PCR

BacteriumOriginResults of PCR with various primer pairsg
rpoBkatGmabA-inhAembBpncArpsLrrsgyrA
M. tuberculosis IMCJ105 clinical isolates from Japan++++++++
M. tuberculosis P33 clinical isolates from Poland++++++++
M. tuberculosis H37RvATCCa 27294++++++++
M. tuberculosis H37RaATCC 25177++++++++
M. bovis BCG Japanese strain 172Japan BCG Laboratoryb++++++++
M. aviumATCC 25291++
M. gastriGTCc 610 (ATCC 15754)+++
M. intracellulareJCMd 6384 (ATCC 13950)++
M. kansassiiJCM 6379 (ATCC 124878)++
M. marinumGTC 616 (ATCC 927)+++
M. simiaeGTC 620 (ATCC 25275)+
M. scrofulaceumJCM 6381 (ATCC 19981)+
M. szulgaiJCM 6383 (ATCC 35799)+++
M. nonchromogenicumJCM 6364 (ATCC 19530)+
M. terraeGTC 623 (ATCC 15755)
M. chelonaeJCM 6390 (ATCC 14472)+
M. fortuitumRIMDe 1317004 (ATCC 6841)+
M. phleiRIMD 1326001 (ATCC 19249)
M. smegmatisATCC 19420+
Escherichia coliATCC 8739
Haemophilus influenzaeIIDf 984 (ATCC 9334)
Klebsiella pneumoniaeIID5209 (ATCC 15380)
Legionella pneumophilaGTC 745
Mycoplasma pneumoniaeIID 817
Pseudomonas aeruginosaATCC 27853
Rhodococcus equiATCC 33710
Staphylococcus aureusN315
Streptococcus pneumoniaeGTC 261
  • a American Type Culture Collection, Rockville, MD.

  • b Japan BCG Laboratory, Tokyo, Japan.

  • c Gifu Type Culture Collection, Department of Microbiology-Bioinformatics, Regeneration and Advanced Medical Science, Gifu University, Graduate School of Medicine, Bacterial Genetic Resources, Gifu, Japan.

  • d Japan Collection of Microorganisms, Institute of Physical and Chemical Research (RIKEN), Saitama, Japan.

  • e Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.

  • f Institute of Medical Science, University of Tokyo, Tokyo, Japan.

  • g Amplification results were determined by agarose gel electrophoresis. Symbols: +, presence of amplification products; −, absence of amplification products.