TABLE 4.

Nucleotide and amino acid changes found in 138 clinical isolates of M. tuberculosisaa

GeneNo. of isolates (n = 138)Isolate originChanges% Resistant (no. of isolates displaying resistance)Other mutations
NucleotideAmino acid (silent mutation)
rpoBb83JapanNoneNone0
26PolandNoneNone0
1JapanTCG→TTGS450L*0
1PolandCAA→CTAQ513L100
1JapanGAC→GTCD516V100
1JapanTCG→TTGS522L100
1JapanCAC→TACH526Y100
2JapanCAC→CGCH526R100
1PolandCAC→ACCH526T100
7JapanTCG→TTGS531L100
5JapanTCG→TGGS531W100
5PolandTCG→TTGS531L100
2JapanAGC→AGG and CAC→CGCS509R* and H526R100
2JapanATG→ATT and GAC→TACM515I and D516Y100
katG14JapanNoneNone0
23PolandNoneNone4.4 (1)
1JapanCTG→TTG(L653L)0
1JapanGCC→ACCA65T*0
1JapanGCG→CTGA245V*0
1PolandCAG→CCGQ295P*100
1PolandGGC→GTCG297V*100
4JapanAGC→ACCS315T100
2JapanAGC→AACS315N100
3PolandAGC→ACCS315T100
1JapanGTC→GCCV725A*0
63JapanCGG→CTGR463L3.2 (2)
2JapanCGG→CTGR463L100−15C→T upstream of mabA
1PolandACC→CCCT324P*100−15C→T upstream of mabA
7JapanAGC→ACC and CGG→CTGS315T and R463L100
2JapanTTG→TTC and CGG→CTGL141F* and R463L100
1JapanATG→ACG and CGG→CTGM257T* and R463L100−5T→A* upstream of mabA
1JapanCTG→CAG and CGG→CTGL48Q* and R463L100−15C→T upstream of mabA
1JapanATG→ACG, CGG→CTG, and GTC→GCCM257T*, R463L and V708P*100−15C→T upstream of mabA
2JapanCTA insertion at position 1170L390 insertion*100
mabA-inhA operonc97JapanNoneNone0
29PolandNoneNone3.4 (1)
2Japan−15C→T upstream of mabANone100R463L in katG
1Poland−15C→T upstream of mabANone100T324P* in katG
1Japan−15C→T upstream of mabANone100L48Q* and R463L in katG
1Japan−15C→T upstream of mabANone100M257T*, R463L and V708P* in katG
1Japan−15C→T upstream of mabANone100
4Poland−15C→T upstream of mabANone100
1Japan−8T→A upstream of mabANone100
1Japan−5T→A* upstream of mabANone100M257T* and R463L in katG
embB77JapanNoneNone0
30PolandNoneNone3.3 (1)
2JapanGAC→GAT(D345D)100 (2)
1JapanGAC→GAT(D534D)0
5JapanATG→GTGM306V100
1JapanATG→ATTM306I100
1JapanATG→ATCM306I100
2PolandATG→ATAM306I100
2JapanGAC→GCCD354A*100
NucleotideAmino acid (silent mutation)
4JapanGAG→GCGE378A0
1JapanGTG→TTGV492L*0
1PolandCAG→CGGQ497R100
2JapanGCC→ACCA680T*0
3JapanGCC→GTCA1007V*0
1JapanGAC→AACD1024N0
2JapanCTG→CTA and GAG→GCG(L355L) and E378A0
1JapanAAT→TAT and ATG→ATAN296Y* and M306I100
2JapanATG→CTG, GAG→GCG, and CCC→CCAM306L, E378A, and (P1075P)100
pncA89JapanNoneNone0
30PolandNoneNone0
2JapanTCC→TCT(S65S)0
1JapanGCG→GAGA3E*100
1PolandCAG→CCGQ10P100
1JapanGAC→GCCD12A100
2JapanCAC→CAAH51Q100
1PolandCAC→CAGH51Q100
3JapanCCG→CTGP54L*100
1JapanTGC→TGGC72W*100
2JapanGGT→AGTG132S100
2JapanATT→ACTI133T100
1PolandCGC→AGCR148S100
1JapanATG→GTGM175V*100
1JapanGAC→AACD53N*100
gyrA7JapanGAG→CAGE21Q*0
11PolandGAG→CAGE21Q*0
95JapanGAG→CAG and AGC→ACCE21Q* and S95T0
22PolandGAG→CAG and AGC→ACCE21Q* and S95T0
1JapanGAG→CAG, GAC→GGC, and AGC→ACCE21Q*, D94G, and S95T100
1JapanGAG→CAG, GCG→GTG, and AGC→ACCE21Q*, A90V, and S95T100
1JapanGAG→CAG, GCG→GTG, GAC→GCC, and AGC→ACCE21Q*, A90V, D94A, and S95T100
rpsL87JapanAAA→AAG(K121K)11.5 (10)
33PolandAAA→AAG(K121K)6.1 (2)
2JapanAAA→AAG(K121K)100516C→T in rrs
1JapanAAA→AAG(K121K)1001,061C insertion* in rrs
1JapanAAA→AAG(K121K)1001,400A→G in rrs
1JapanAAA→AAG(K121K)1001,400A→G and 1539A→G in rrs
13JapanAAG→AGG and AAA→AAGK43R and (K121K)100
rrs33PolandNone6.1 (2)(K121K) in rpsL
87JapanNone11.5 (10)(K121K) in rpsL
13JapanNone100K43R and (K121K) in rpsL
2Japan516C→T100(K121K) in rpsL
1Japan1,061C insertion*100(K121K) in rpsL
1Japan1,400A→G100(K121K) in rpsL
1Japan1,400A→G and 1539A→G100(K121K) in rpsL
  • a *, mutation not previously reported.

  • b The codon numbering system of RpoB initially described by Telenti et al. (31) was used. The codon numbers of RpoB are designated on the basis of alignment of translated E. coli rpoB sequence with a portion of translated M. tuberculosis sequence and are not the positions of the actual M. tuberculosis rpoB codons.

  • c Nucleotide numbering based on nucleotide position relative to mabA start codon.